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201: can get that from notes, week 2. 202. Describe the power of site directed mutagenesis when compared to random mutagenesis Site directed mutagenesis allows us to make changes at the codons we want to, to test out exactly which parts of the protein contribute to its function and/or structure. Why is it important to use a dut, ung e.coli strain to generate ss DNA for site-directed mutagenesis? Because the DNA will have U’s incorporated, which will be chopped up when we transform wild-type cells with the DNA, so we can get mutation on both strands (look at week 4 notes). Genotype of E.coli strain is hsdS, hsdR. Can it restrict or modify DNA? No to both, because hsdS is nonfunctional, so even though it has functional hsdM, there’s no specificity. 205. look at notes for week 4. 206. Look at notes for week 4. Answer is 42=16. 207. Look at notes for week 4. 208. Look at notes for week 4. |
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