For The Bernal Lab

Standard Operating Procedures

_____________________________________________

• 20X-Borax Solution
• Agarose Gels
• Antibiotic Stocks
• BCA Calculator
• Bradford Assay
• CaCl2 Competent Cells
• Coomassie Blue Staining
• Electrophoresis DNA DEAE Purification
• Flag Agarose Protocol
• Magic-Media
• Making Grids from Scratch
• Mammalian Cell Transfection
• Micro Bradford Assay
• Native-PAGE and Western Blot
• Peptide Conjugation for Antibodies
• Phosphate Buffer
• Protein A/G/L Affinities
• Protein Gels
• Reversible Staining of SDS-PAGE gels
• Silver Staining Gels
• Sodium/Potassium Acetate Buffer
• Studier Magic Media
• Western Blot